Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).

Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).

Appl Plant Sci. 2013 Nov;1(11)

Authors: Ison JL, Wagenius S, Reitz D, Ashley MV

Abstract

UNLABELLED: •

PREMISE OF THE STUDY: Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. •

METHODS AND RESULTS: A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy-Weinberg equilibrium in three populations with varying numbers of plants. The loci had between three and 14 alleles and collectively provided high paternity exclusion probabilities. •

CONCLUSIONS: These sets of microsatellite primers will provide researchers and land managers with valuable information on the population genetic structure and gene flow between fragmented prairie populations.

PMID: 25202499 [PubMed]